Name | CM-H2DCFDA |
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Description | CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive[1]. |
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Related Catalog | |
In Vitro | Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).ROS Measurement:1. Incubate the cells according to your normal protocol.2. Treat cells with 10 μM CM-H2DCFDA for 30 min at 37°C in darkness.3. Wash the excess probe.4. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. |
References |
[1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11. |
Molecular Formula | C27H19Cl3O8 |
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Molecular Weight | 577.79 |