2758364-03-1
2758364-03-1 structure
- Name: Rf470DL
- Chemical Name: Rf470DL
- CAS Number: 2758364-03-1
- Molecular Formula: C26H31ClN4O5S
- Molecular Weight: 547.07
- Catalog: Signaling Pathways Anti-infection Bacterial
- Create Date: 2022-10-12 08:07:16
- Modify Date: 2024-01-06 19:30:49
-
Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm)[1].
| Name | Rf470DL |
|---|
| Description | Rf470DL is a rotor-fluorogenic D-amino acid (RfDAA). Rf470DL can be used for labeling bacteria (Ex=470 nm, Em=640 nm)[1]. |
|---|---|
| Related Catalog | |
| In Vitro | Rf470DL can realize ash-free imaging of bacterial cell walls[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. RfDAA/FDAA stock solutions are prepared in DMSO at a concentration of 100 mM and stored at -20°C before use. 2.1 For long-pulse labeling of B. subtilis and E. coli, dilute the exponential phase cultures with fresh LB broth containing 1 mM Rf470DL to OD600~0.05, and incubate for 1 h. 2.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation. 3.1 For short-pulse labeling of S. venezuelae, Rf470DL stock solution is added directly to exponential phase cultures to a final concentration of 0.5 mM, followed by incubating at 30 °C with shaking for 15 min. 3.2 Image immediately using a Nikon Ti-E inverted microscopy system without washing and fixation. |
| References |
| Molecular Formula | C26H31ClN4O5S |
|---|---|
| Molecular Weight | 547.07 |