| Name | FDGlcU |
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| Description | FDGlcU can be used as a fluorescent probe for non-invasively image with a high level of fluorescent activity. FDGlcU is non-fluorescent when the fluorescein is conjugated with two mono-glucuronides (Ex/Em=480/514 nm)[1]. |
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| Related Catalog | |
| In Vitro | FDGlcU (0.5 μg/mL; 12 h) shows enzymatic activity and inhibits βG activity in live bacteria[1]. |
| In Vivo | FDGlcU (7.3 μM/kg) can be used for in vivo time-lapse imaging of bacterial βG activity[1]. FDGlcU can be used for real-time imaging in vivo[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Whole-body imaging for intestinal βG activity[1]: 1. On the day of imaging, gavage mice with 50 μL of DDW, FDGlcU (7.3 μM/kg), and fluorescein (7.3 μM/kg). 2. Anesthetize mice with isoflurane and whole-body optical images using an IVIS spectrum optical imaging system with a GFP filter set. 3. In biodistribution study, mice are sacrificed 3 hour after gavaged with FDGlcU or fluorescein (λex =480 nm, λem =514 nm). |
| References |
| Molecular Formula | C39H28F5NO18 |
|---|---|
| Molecular Weight | 893.63 |