172646-19-4
172646-19-4 structure
- Name: Calcium Orange AM
- Chemical Name: Calcium Orange AM
- CAS Number: 172646-19-4
- Molecular Formula: C59H62N6O21S
- Molecular Weight: 1223.22
- Catalog: Research Areas Others
- Create Date: 2022-10-15 10:43:28
- Modify Date: 2024-04-03 09:28:05
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Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles[1].
| Name | Calcium Orange AM |
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| Description | Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles[1]. |
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| Related Catalog | |
| In Vitro | Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Calcium Orange AM is freshly prepared in dehydrated DMSO before each experiment. 2. Prepare the recording medium for measuring [Ca2+]i in cells consisted of 20 mM HEPES, 115 mM NaCl, 5.4 mM KCl, 0.8 mM MgCl2, 1.8 mM CaCl2, and 13.8 mM D-glucose adjusted to pH 7.4 by adding 1 M KOH. 3. Exchange the culture medium for 1 mL of a recording medium including 10 μM Calcium Orange AM. 4. Incubate cells under conditions of 5% CO2 and 37°C for 40 min. 5. Remove the recording medium, including calcium orange AM from the dish, and add the culture medium after washing it three times with 1 mL of the recording medium. 6. Incubate the cells for 1 h. After incubation, exchange the culture medium for the recording medium again. 7. Detect fluorescence. |
| References |
| Molecular Formula | C59H62N6O21S |
|---|---|
| Molecular Weight | 1223.22 |