In Vitro |
Anticancer agent 47 (compound 4j) shows antiproliferative activities with IC50s of 1.6, 0.72, 7.07 µM for HepG2, A549, H596 cells, respectively[1]. Anticancer agent 47 (0.8, 1.6, 3.2 µM; 24 h) induces apoptosis and cell cycle arrest at G0/G1 phase[1]. Anticancer agent 47 (5 µM; 5h) significantly increases ROS production[1]. Apoptosis Analysis[1] Cell Line: HepG2, H596 cells Concentration: 0.8, 1.6, 3.2 µM Incubation Time: 24 h Result: Induced apoptosis with the apoptotic cell rates were 14.23, 20.47 and 27.66% at 0.8, 1.6, 3.2 µM in HepG2 cell, respectively. Cell Cycle Analysis[1] Cell Line: HepG2 cells Concentration: 0.8, 1.6, 3.2 µM Incubation Time: 24 h Result: Showed 48.54%, 49.60% and 53.00% cells were at G0/G1 phase at 0.6, 1.2 and 2.4 µM, respectively.
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