921765-76-6

921765-76-6 structure

Name: 3x DYKDDDDK Tag
Chemical Name: 3x DYKDDDDK Tag
CAS Number: 921765-76-6
Molecular Formula: C₁₂₃H₁₇₆N₃₀O₅₈
Molecular Weight: 3002.88
Catalog: Research Areas Others
Create Date: 2022-01-18 15:36:02
Modify Date: 2024-02-11 12:04:41
DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2].

Name	3x DYKDDDDK Tag

Description	DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].
References	[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830. [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

Molecular Formula	C₁₂₃H₁₇₆N₃₀O₅₈
Molecular Weight	3002.88