1403838-79-8
1403838-79-8 structure
- Name: BRD0539
- Chemical Name: BRD0539
- CAS Number: 1403838-79-8
- Molecular Formula: C25H25FN2O3S
- Molecular Weight: 452.54
- Catalog: Signaling Pathways Cell Cycle/DNA Damage CRISPR/Cas9
- Create Date: 2020-08-08 09:43:07
- Modify Date: 2025-08-26 13:41:38
-
BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay[1].
| Name | BRD0539 |
|---|
| Description | BRD0539 is a cell-permeable and non-toxic inhibitor of CRISPR-Cas9. BRD0539 inhibits Streptococcus pyogenes Cas9 (SpCas9) (apparent IC50=22 μM) in an in vitro DNA cleavage assay[1]. |
|---|---|
| Related Catalog | |
| Target |
IC50: 22 μM (SpCas9)[1] |
| In Vitro | BRD0539 dose-dependently blocks the formation of the DNA-bound state in a dose-dependent fashion. BRD0539 impairs the perturbation induced by the 4PAM DNA[1]. BRD0539 does not interfere with the SpCas9:gRNA interaction[1]. BRD0539 is able to inhibit SpCas9 in the eGFP-disruption assay, BRD0539 is unable to inhibit FnCpf1, a structurally different CRISPR-associated nuclease, in the same assay, further highlighting the specificity of BRD0539[1]. |
| References |
| Molecular Formula | C25H25FN2O3S |
|---|---|
| Molecular Weight | 452.54 |