2024548-03-4

2024548-03-4 structure

Name: Lin28-let-7a antagonist 1
Chemical Name: Lin28-let-7a antagonist 1
CAS Number: 2024548-03-4
Molecular Formula: C₃₁H₂₉N₅O₇
Molecular Weight: 583.59
Catalog: Signaling Pathways Epigenetics MicroRNA
Create Date: 2019-02-08 18:05:38
Modify Date: 2024-01-09 10:13:37
Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction.

Name	Lin28-let-7a antagonist 1

Description	Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction.
Related Catalog	Signaling Pathways >> Epigenetics >> MicroRNA Research Areas >> Others
Target	IC50：4.03 μM (Lin28A-let-7a-1)[1].
In Vitro	Lin28-let-7a antagonist 1 (compound 1) shows a clear antagonistic effect against the Lin28A-let-7a-1 interaction with an IC50 of 4.03 μM. Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency. Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA. When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28[1].
References	[1]. Lim D, et al. Discovery of a Small-Molecule Inhibitor of Protein-MicroRNA Interaction Using Binding Assay with a Site-Specifically Labeled Lin28. J Am Chem Soc. 2016 Oct 7.