1443138-53-1

1443138-53-1 structure

Name: GSK-2200150A
Chemical Name: GSK2200150A
CAS Number: 1443138-53-1
Molecular Formula: C₂₀H₂₃NO₃S
Molecular Weight: 357.466
Catalog: Signaling Pathways Anti-infection Bacterial
Create Date: 2018-09-30 08:16:23
Modify Date: 2025-08-25 23:32:10
GSK2200150A, identified by high-throughput screening (HTS) campaign, is an anti-tuberculosis (TB) agent.

Name	GSK2200150A
Synonyms	Spiro[piperidine-4,4'-[4H]thieno[3,2-c]pyran], 1-[(2,3-dihydro-1,4-benzodioxin-6-yl)methyl]-6',7'-dihydro- 1-(2,3-Dihydro-1,4-benzodioxin-6-ylmethyl)-6',7'-dihydrospiro[piperidine-4,4'-thieno[3,2-c]pyran]

Description	GSK2200150A, identified by high-throughput screening (HTS) campaign, is an anti-tuberculosis (TB) agent.
Related Catalog	Signaling Pathways >> Anti-infection >> Bacterial Research Areas >> Infection
Target	MIC: 0.38 μM (M.tuberculosis strain H37Rv)[1]
In Vitro	GSK2200150A is a novel antimycobacterial agent against Mycobacterium tuberculosis.The activities of GSK2200150A containing the spirocycle core are determined against the virulent M.tuberculosis strain (H37Rv) with MIC of 0.38 μM[1].
Cell Assay	GSK2200150A is tested for activity at either single concentration (100 µM) or serially diluted in 10 µL of purified H2O in triplicate in 96 well microtiter plates. M. tuberculosis H37Rv is grown in complete Middlebrook 7H9 media containing albumin, dextrose and catalase (ADC), 20% Tween 80 and 50% glycerol. A bacterial suspension (90 µL) at OD600 nm of 0.001 is added to the wells and incubated for 7 days. Resazurin (10 µL; 0.05%(w/v)) is then added, incubated for 24 h at 37°C, and fluorescence measured at 590 nm using a FLUOstar Omega microplate reader. After subtraction of background fluorescence from all wells, the percentage mycobacterial survival is determined by comparing the fluorescence of wells containing compounds compared to control wells not treated with compound[1].
References	[1]. Badiola KA, et al. Efficient Synthesis and Anti-Tubercular Activity of a Series of Spirocycles: An Exercise in OpenScience. PLoS One. 2014 Dec 10;9(12):e111782.