144707-18-6
144707-18-6 structure
- Name: 2-D08
- Chemical Name: 2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one
- CAS Number: 144707-18-6
- Molecular Formula: C15H10O5
- Molecular Weight: 270.237
- Catalog: Signaling Pathways Metabolic Enzyme/Protease E1/E2/E3 Enzyme
- Create Date: 2018-07-19 08:38:49
- Modify Date: 2024-01-06 11:15:39
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2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM.
| Name | 2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one |
|---|---|
| Synonyms |
4H-1-Benzopyran-4-one, 2-(2,3,4-trihydroxyphenyl)-
2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one MFCD27995567 |
| Description | 2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM. |
|---|---|
| Related Catalog | |
| Target |
IC50: 0.49 nM (Axl)[2] |
| In Vitro | 2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[1]. 2-D08 decreases the ratio of p-Axl to t-Axl in a dose-dependent manner. Suppression of Axl kinase activity by 2D08 disrupts the cytoskeleton and actin filaments with re-organization at cellular junctions[2]. |
| Cell Assay | Human lung multi-potent cells at passage 5 are incubated with DMEM+0.5% BSA+penicillin/streptomycin containing either 0.1% DMSO (vehicle) or 2D08 (10 μM) on Permanox culture slides for 6 days. Cells are fixed with 4% PFA for 30 min and then blocked and permeabilized with 10% goat serum and 0.3% Triton-X 100 in PBS for 30 min[2]. |
| Animal Admin | Mice[3] Eight- to 10-week-old mice (both male and female) are used for the study. A 5 cm-long glass pipette is buffered with Mineral Oil and then attached to the injection apparatus to take up 10 μL of 2-D08 (30 μM) or NaCl[3]. |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 517.9±50.0 °C at 760 mmHg |
| Molecular Formula | C15H10O5 |
| Molecular Weight | 270.237 |
| Flash Point | 201.7±23.6 °C |
| Exact Mass | 270.052826 |
| LogP | 2.77 |
| Vapour Pressure | 0.0±1.4 mmHg at 25°C |
| Index of Refraction | 1.732 |
| Storage condition | -20℃ |
