DC Chemicals Limited
China
Product Name: SKL2001
Price: $400.0/100mg $700.0/250mg $1300.0/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

909089-13-0

909089-13-0 structure

909089-13-0 structure

Name: SKL2001
Chemical Name: 5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide
CAS Number: 909089-13-0
Molecular Formula: C₁₄H₁₄N₄O₃
Molecular Weight: 286.286
Catalog: Signaling Pathways Stem Cell/Wnt Wnt
Create Date: 2018-07-19 22:01:18
Modify Date: 2024-01-05 12:30:54
SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity.

Name	5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide
Synonyms	5-(2-Furyl)-N-[3-(1H-imidazol-1-yl)propyl]-1,2-oxazole-3-carboxamide 3-Isoxazolecarboxamide, 5-(2-furanyl)-N-[3-(1H-imidazol-1-yl)propyl]- SKL2001

Description	SKL2001 is an agonist of the Wnt/β-catenin pathway, with anti-cancer activity.
Related Catalog	Signaling Pathways >> Stem Cell/Wnt >> β-catenin Signaling Pathways >> Stem Cell/Wnt >> Wnt Research Areas >> Cancer
Target	Wnt/β-catenin[1]
In Vitro	SKL2001 is an agonist of the Wnt/β-catenin pathway, and also upregulates the expression of Axin2, a downstream target of the Wnt/β-catenin pathway, but shows no effect on NF-κB, p53 reporter activity and GSK-3β activity. SKL2001 causes osteoblast differentiation (20 and 40 μM) and suppresses preadipocyte differentiation (5, 10, and 30 μM) via the activation of the Wnt/β-catenin pathway. SKL2001 (5, 10, and 30 μM) stabilizes intracellular β-catenin in 3T3-L1 cells[1]. SKL2001 (40 μM) significantly inhibits the proliferation of HCT116 spheroids independently of cytotoxicity and the inhibition is reversible; SKL2001 cuases cell cycle arrest in HCT116 spheroids. SKL2001 (40 μM) enhances round-shape spheroid formation and E-cadherin expression[2].
Cell Assay	ST2 cells are cultured on glass chamber slides and then treated with DMSO or SKL2001 for 15 h. After treatment, the cells are washed with PBS, fixed with 4% formaldehyde, permeabilized in 0.3% Triton X-100, and blocked in 4% bovine serum albumin for 1 h. The cells are stained with anti-β-catenin antibody and then analyzed by confocal microscopy using a microscope[1].
References	[1]. Gwak J, et al. Small molecule-based disruption of the Axin/β-catenin protein complex regulates mesenchymal stem cell differentiation. ell Res. 2012 Jan;22(1):237-47. [2]. Ohashi W, et al. SKL2001 suppresses colon cancer spheroid growth through regulation of the E-cadherin/β-Catenin complex. Biochem Biophys Res Commun. 2017 Nov 25;493(3):1342-1348.

Density	1.4±0.1 g/cm3
Boiling Point	595.7±50.0 °C at 760 mmHg
Molecular Formula	C₁₄H₁₄N₄O₃
Molecular Weight	286.286
Flash Point	314.1±30.1 °C
Exact Mass	286.106598
LogP	0.37
Vapour Pressure	0.0±1.7 mmHg at 25°C
Index of Refraction	1.655
Storage condition	-20℃