137993-41-0

137993-41-0 structure
137993-41-0 structure
  • Name: Rhodamine 800
  • Chemical Name: Rhodamine 800
  • CAS Number: 137993-41-0
  • Molecular Formula: C26H26ClN3O5
  • Molecular Weight: 495.955
  • Catalog: Dye Reagents
  • Create Date: 2018-02-07 02:35:57
  • Modify Date: 2025-08-22 15:19:25
  • Rhodamine 800 is a near-infrared fluorescent dye.

Name Rhodamine 800
Synonyms 9-Cyano-2,3,6,7,12,13,16,17-octahydro-1H,5H,11H,15H-pyrido[3,2,1-ij]quinolizino[1',9':6,7,8]chromeno[2,3-f]quinolin-4-ium perchlorate
Description Rhodamine 800 is a near-infrared fluorescent dye.
Related Catalog
In Vitro Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak[1].
Kinase Assay Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2].
References

[1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

[2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.

Molecular Formula C26H26ClN3O5
Molecular Weight 495.955
Exact Mass 495.156097
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR NONH for all modes of transport
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