- Nanjing Peptide Biotech Ltd
- China
- Product Name: RHOD-2,AM
- Price: ￥Inquiry/10g ￥Inquiry/100g ￥Inquiry/1kg ￥Inquiry/10kg
- Purity: 98.0%
- Stocking Period: 7 Day
- Contact: jom
- Dayang Chem (Hangzhou) Co., Ltd.
- China
- Product Name: RHOD-2, AM
- Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Ms Wang
129787-64-0
129787-64-0 structure
- Name: Rhod-2 AM
- Chemical Name: rhod-2, am
- CAS Number: 129787-64-0
- Molecular Formula: C51H57BrN4O19
- Molecular Weight: 1109.92
- Catalog: Dye Reagents
- Create Date: 2018-02-27 08:00:00
- Modify Date: 2024-01-02 08:37:12
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Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm).
| Name | rhod-2, am |
|---|---|
| Synonyms |
xanthylium
5-tetramethylrhodamine isothiocyanate rhod 2 tetramethylrhodamine-5-isothiocyanate 5-tritc Rhod-2 AM |
| Description | Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). |
|---|---|
| Related Catalog | |
| In Vitro | A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i.[1]. |
| Cell Assay | For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1]. |
| References |
| Molecular Formula | C51H57BrN4O19 |
|---|---|
| Molecular Weight | 1109.92 |
| PSA | 254.73000 |
| LogP | 0.94060 |