273221-67-3
273221-67-3 structure
- Name: Fluo-4 AM
- Chemical Name: Fluo 4AM
- CAS Number: 273221-67-3
- Molecular Formula: C51H50F2N2O23
- Molecular Weight: 1096.94000
- Catalog: Dye Reagents
- Create Date: 2017-09-29 06:53:36
- Modify Date: 2025-08-27 13:49:28
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Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).
| Name | Fluo 4AM |
|---|---|
| Synonyms | Fluo-4 AM |
| Description | Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). |
|---|---|
| Related Catalog | |
| In Vitro | Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1]. |
| Cell Assay | For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 mM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1]. |
| References |
| Density | 1.47 |
|---|---|
| Molecular Formula | C51H50F2N2O23 |
| Molecular Weight | 1096.94000 |
| Exact Mass | 1096.28000 |
| PSA | 301.08000 |
| LogP | 4.37660 |