ESTRADIOL HEMIHYDRATE

Names

[ Name ]:
ESTRADIOL HEMIHYDRATE

[Synonym ]:
(17β)-Estra-1,3,5(10)-triene-3,17-diol hydrate (2:1)
ESTRADIOL HEMIHYDRATE
cxy7b3q98z
Estra-1,3,5(10)-triene-3,17-diol, (17β)-, hydrate (2:1)
Estra-1,3,5(10)-triene-3,17β-diol hydrate (2:1)
unii-cxy7b3q98z

Biological Activity

[Description]:

Estradiol (β-Estradiol) hemihydrate is a steroid hormone and the major female sex hormone. Estradiol (β-Estradiol) hemihydrate can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol (β-Estradiol) hemihydrate can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering[1][2].

[Related Catalog]:

Signaling Pathways >> Others >> Estrogen Receptor/ERR

Research Areas >> Cancer

Research Areas >> Neurological Disease

[In Vitro]

Estradiol hemihydrate (10 nM, 7 days) induces neural differentiation and increased neurite branching of human endometrial stem cells (hEnSCs)[1]. Estradiol hemihydrate (17β-estradiol, 10 nM, 7 days) increases the expression of neuron-like cell markers (Tuj-1, nestin and NF-H) in neural-like cells differentiated from hEnSCs[1]. Cell Differentiation Assay[1] Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue Concentration: 10 nM Incubation Time: 7 days Result: Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively. Immunofluorescence[1] Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue Concentration: 10 nM Incubation Time: 7 days Result: Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

[In Vivo]

Estradiol hemihydrate (1 nM, the hippocampal slices from FBN-ARO-KO mice) rescues long-term potentiation (LTP) amplitude[1]. Estradiol hemihydrate (0.0167 mg, implanted s.c., FBN-ARO-KO mice) rescues the molecular and functional deficits in FBN-ARO-KO mice[1]. Animal Model: FBN-ARO-KO Mice[2] Dosage: 1 nM Administration: Treated for the hippocampal slices Result: Rescued long-term potentiation (LTP) amplitude of both male and female mice. Animal Model: FBN-ARO-KO Mice[2] Dosage: 0.0167 mg Administration: Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation. Result: Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain. Rescued the spatial learning and memory defects.

[References]

[1]. Elham Hasanzadeh. Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells. Cell Biol Int. 2021 Jan;45(1):140-153.

[2]. Yujiao Lu, et al. Neuron-Derived Estrogen Regulates Synaptic Plasticity and Memory. J Neurosci. 2019 Apr 10;39(15):2792-2809.

Chemical & Physical Properties

[ Boiling Point ]:
445.9ºC at 760mmHg

[ Molecular Formula ]:
C₁₈H₂₆O₃

[ Molecular Weight ]:
562.779

[ Flash Point ]:
209.6ºC

[ Exact Mass ]:
562.365845

[ PSA ]:
49.69000

[ LogP ]:
3.54490

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H351-H360Fd-H362

[ Precautionary Statements ]:
P201-P263-P281-P308 + P313

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type P2 (EN 143) respirator cartridges

[ Hazard Codes ]:
T

[ Risk Phrases ]:
60-63-40-64

[ Safety Phrases ]:
53-36/37-45

[ RIDADR ]:
NONH for all modes of transport

Articles

Substrate competition studies using whole-cell accumulation assays with the major tripartite multidrug efflux pumps of Escherichia coli.

Antimicrob. Agents Chemother. 51 , 923-9, (2007)

AcrAB-TolC is the major, constitutively expressed tripartite multidrug efflux system in Escherichia coli that recognizes various structurally unrelated molecules, including many antibiotics, dyes, and...

Development of a novel magnetic molecularly imprinted polymer coating using porous zeolite imidazolate framework-8 coated magnetic iron oxide as carrier for automated solid phase microextraction of estrogens in fish and pork samples.

J. Chromatogr. A. 1365 , 35-44, (2014)

A high-performance magnetic molecularly imprinted polymer (MIP) coating using zeolite imidazolate framework-8 coated magnetic iron oxide (Fe3O4@ZIF-8) as a carrier was developed for simultaneous autom...

Cheminformatics analysis of assertions mined from literature that describe drug-induced liver injury in different species.

Chem. Res. Toxicol. 23 , 171-83, (2010)

Drug-induced liver injury is one of the main causes of drug attrition. The ability to predict the liver effects of drug candidates from their chemical structures is critical to help guide experimental...