ATP-Red 1
Names
[ CAS No. ]:
1847485-97-5
[ Name ]:
ATP-Red 1
Biological Activity
[Description]:
ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells.
[Related Catalog]:
[In Vitro]
ATP-Red 1 is a multisite-binding switchable fluorescent probe, and can selectively and rapidly responds to intracellular concentrations of ATP in living cells. The maximum absorption and emission wavelength of are 570/566 nm and 590/585 nm. ATP-Red 1 has good membrane permeability, and in the presence of 5 mM ATP, the fluorescence intensity of ATP-Red 1 increases 5.6-fold. ATP-Red 1 (2.5 μM, 20 min) shows much weaker fluorescence after KCN-induced inhibition of OXPHOS, which results in reduced mitochondrial ATP levels in OSCC cells[1].
[Cell Assay]
OSCC Cells are plated in 96-well flat-bottomed plates at 1×105 cells per well and allowed to grow 3 or 24 h prior to exposure to ATP-Red 1. Then MTT reagent is added for 4 h at 37 °C and DMSO (100 μL/well) is further incubated with cells for 15 min after removing the medium. The absorbance at 570 nm and 690 nm (background signal) is recorded in a Spectra Max M2 microplate reader. The following formula is used to calculate the viability of cell growth: Cell viability (%) = (mean of A value of treatment group / mean of A value of control) × 100[1].
[References]
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Chemical & Physical Properties
[ Molecular Formula ]:
C34H36BN3O4
[ Molecular Weight ]:
561.48
[ Storage condition ]:
2-8℃