cypate

Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging[1][2].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. 将每种类型细胞在单独的 35 毫米玻璃底培养皿上接种 1×106 个。 2. 24 小时后，将细胞与 10 µM Cypate 无酚红培养基溶液一起孵育 2 小时。 3. 处理后，细胞用 PBS 洗涤 3 次并用 4% 多聚甲醛固定用于成像，或收集细胞内的 Cypate 内容物用于光谱和/或 LC-MS 分析。 4. 对体外细胞培养实验进行共聚焦显微镜检查。Cypate 的激发波长为 647 nm[1]。

Cypate (10 nmol; 100 µL; 静脉给药; 每 24 小时一次; 连续 6 天) 导致肝脏蓄积最高[1]。 Cypate (5 mg/kg; 静脉给药) 在具有 4T1 细胞的 Balb/c 小鼠肿瘤中的荧光信号微弱并迅速衰减，这可能是由于游离 Cypate 从体内快速消除所致。在游离cypate组中观察到肝脏和肾脏中更强的荧光。使用 IVIS Lumina 成像系统 (Ex-745 nm; Em-800 nm) 评估 Cypate 荧光信号[2]。 Animal Model: Foxn1nu/Foxn1nu nude 2.5-month-old female mice with breast cancer cells (MDA-MB-231 Luc2)[1] Dosage: 10 nmol in 100 µL PBS Administration: IV; every 24 hours for 6 days Result: The accumulation alone in the tumor was negligible for 24 hours after the injection. The liver had the highest accumulation at all time points.

[1]. Mona Doshi, et al.  Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging. Mol Pharmacol. 2019 May;95(5):475-489.  

[2]. Yuanyuan Li, et al. Light-Decomposable Polymeric Micelles with Hypoxia-Enhanced Phototherapeutic Efficacy for Combating Metastatic Breast Cancer. Pharmaceutics. 2022 Jan 21;14(2):253.