L82-G17

Names

[ Name ]:
L82-G17

[Synonym ]:
4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]-3(2H)-pyridazinone
Benzaldehyde, 3-hydroxy-, 1-[2-(5-chloro-1,6-dihydro-6-oxo-4-pyridazinyl)hydrazone]
4-Chloro-5-[(2Z)-2-(3-hydroxybenzylidene)hydrazino]pyridazin-3(2H)-one

Biological Activity

[Description]:

L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity[1].

[Related Catalog]:

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis

[In Vitro]

L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI[1]. L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding[1]. L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation[1]. L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage[1]. Cell Viability Assay[1] Cell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 5 days Result: Reduced cell number by about 70% at 20 μM. Cell Proliferation Assay[1] Cell Line: CH12F3 WT and CH12F3Δ/Δ cells Concentration: 0-100 μM Incubation Time: 72 h Result: Had great effect on the proliferation and survival of the parental CH12F3 cells.

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Molecular Formula ]:
C₁₁H₉ClN₄O₂

[ Molecular Weight ]:
264.668

[ Exact Mass ]:
264.041412

[ LogP ]:
0.99

[ Index of Refraction ]:
1.685

Related Compounds

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