thermolysin fromGeobacillus stearothermophilus

Names

[ Name ]:
thermolysin fromGeobacillus stearothermophilus

[Synonym ]:
MFCD00165549

Biological Activity

[Description]:

Thermolysin is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues[1].

[Related Catalog]:

Research Areas >> Infection

[In Vitro]

Thermolysin (100 μg in 1 mL, 64°C for 4 h, in isolated starch granules) digestion results in the selective removal of a group of low-molecular-mass proteins ranging between 10 and 27 kD[2]. Thermolysin can be used for the hydrolysis of bovine liver sarcoplasmic proteins[3].

[In Vivo]

Thermolysin (50 mg/kg, p.o., once) lowers systolic blood pressure in SHR rats[4]. Thermolysin is not acutely toxic with an oral LD50 of more than 18000 mg/kg in rats and more than 24000 mg/kg in mice (Acute toxicity studies)[5]. Animal Model: Spontaneously hypertensive rats (SHR) rats[4] Dosage: 50 mg/kg Administration: Oral administration (p.o.), once Result: Lowered systolic blood pressure in SHR rats, with maximal reduction by 22 mm in systolic blood pressure being observed 6 h after administration.

Chemical & Physical Properties

[ Storage condition ]:
20°C

Safety Information

[ Symbol ]:

GHS07, GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H315-H317-H319-H334-H335

[ Precautionary Statements ]:
P261-P280-P305 + P351 + P338-P342 + P311

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ Hazard Codes ]:
Xn

[ Risk Phrases ]:
36/37/38-42/43

[ Safety Phrases ]:
22-26-36/37

[ RIDADR ]:
NONH for all modes of transport

Articles

Enzymatic amine acyl exchange in peptides on gold surfaces.

Angew. Chem. Int. Ed. Engl. 51(52) , 13016-8, (2012)

Reversible as well as stereo- and chemoselective: various proteases such as thermolysin and chymotrypsin catalyze amine acyl exchange in peptides. This acyl exchange can be used to modify amino-functi...

Local unfolding is required for the site-specific protein modification by transglutaminase.

Biochemistry 51 , 8679-8689, (2012)

The transglutaminase (TGase) from Streptomyces mobaraensis catalyzes transamidation reactions in a protein substrate leading to the modification of the side chains of Gln and Lys residues according to...

Angiotensin-I converting enzyme inhibitory and antioxidant activities of egg protein hydrolysates produced with gastrointestinal and nongastrointestinal enzymes.

J. Food Sci. 76(6) , C801-7, (2011)

Egg is a well-known rich source of bioactive peptides. In this study, egg protein (egg white and egg yolk proteins) hydrolysates were produced with gastrointestinal enzymes (pepsin and pancreatin) or ...

Related Compounds

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