Polyethylene-polypropylene glycol

Names

[ Name ]:
Polyethylene-polypropylene glycol

[Synonym ]:
3-Acetoxy-2,2-bis(acetoxymethyl)propyl acetate
Poloxamer 407
MFCD00082049
Pentaerythrityl Tetraacetate
PAG
Poloxamer
2,2-Bis[(acetyloxy)methyl]-1,3-propanediol Diacetate
1,3-Propanediol, 2,2-bis[(acetyloxy)methyl]-, diacetate
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)
Tetra-O-acetylpentaerythritol
pentaerythritol tetraacetate

Biological Activity

[Description]:

Poloxamer 407 is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8™ AM and Quest Rhod-4™ AM, etc. to improve their water solubility.

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 1 g of Poloxamer 407 in 10 mL distilled water to make a 10% (w/v) stock solution, or 2 g of Poloxamer 407 in 10 mL DMSO to make a 20% (w/v) stock solution. These may require heating from 40 to 50°C for about 30 minutes. Store solution at room temperature. Do not refrigerate or freeze the Poloxamer 407 solution since it may precipitate. If precipitation is observed, the precipitates can be dissolved by heating to 37°C and vortexing before use. 2. Dilute the 10% or 20% Poloxamer 407 stock solution into the cell-loading buffer such as Hanks and 20 mM Hepes buffer (HHBS) at 1:1000 to 1:500 dilution to achieve a 0.02 to 0.04% working solution. 3. The DMSO stock solution of AM ester is then diluted into the 0.02 to 0.04% working solution to achieve a final AM ester concentration between 1 μM and 10 μM. The final concentration of Poloxamer 407 is normally kept at or below 0.08%. 4. The cells are incubated at a desired temperature for between 10 minutes and 1 hour. In general it is desirable to use the minimum amount of AM ester needed to achieve adequate fluorescence signal to noise levels. 5. After labeling, the cells are washed with HHBS or fresh medium before starting the experiment.

[References]

[1]. Antunes FE, et al. Gels of Pluronic F127 and nonionic surfactants from rheological characterization to controlled drug permeation. Colloids Surf B Biointerfaces. 2011 Oct 1;87(1):42-8.

Chemical & Physical Properties

[ Density]:
1.2±0.1 g/cm3

[ Boiling Point ]:
370.7±37.0 °C at 760 mmHg

[ Melting Point ]:
60-50ºC

[ Molecular Formula ]:
C₁₃H₂₀O₈

[ Molecular Weight ]:
304.293

[ Flash Point ]:
160.5±26.5 °C

[ Exact Mass ]:
304.115814

[ PSA ]:
25.06000

[ LogP ]:
0.00

[ Vapour density ]:
>1 (vs air)

[ Vapour Pressure ]:
0.0±0.8 mmHg at 25°C

[ Index of Refraction ]:
1.452

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
H2O: at <70 °Csoluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: TP6000000

CHEMICAL NAME :: Pluronic F98

CAS REGISTRY NUMBER :: 9003-11-6

LAST UPDATED :: 199710

DATA ITEMS CITED :: 7

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 175 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: MIVRA6 Microvascular Research. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1968- Volume(issue)/page/year: 8,320,1974 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M3966 No. of Facilities: 6846 (estimated) No. of Industries: 44 No. of Occupations: 37 No. of Employees: 38016 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X7313 No. of Facilities: 59555 (estimated) No. of Industries: 299 No. of Occupations: 176 No. of Employees: 1005983 (estimated) No. of Female Employees: 296376 (estimated)

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
T

[ Risk Phrases ]:
21-23/24/25-25-36/37/38

[ Safety Phrases ]:
23-24/25-45-36/37/39-26

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
TP6164333

[ HS Code ]:
39059100

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