4-Methylumbelliferyl-β-D-glucuronide hydrate

Names

[ CAS No. ]:
881005-91-0

[ Name ]:
4-Methylumbelliferyl-β-D-glucuronide hydrate

[Synonym ]:
(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(4-methyl-2-oxochromen-7-yl)oxyoxane-2-carboxylic acid,hydrate

Biological Activity

[Description]:

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].

[Kinase Assay]

GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1].

[References]

[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.

[Related Small Molecules]

Chemical & Physical Properties

[ Molecular Formula ]:
C₁₆H₁₈O₁₀

[ Molecular Weight ]:
370.30800

[ Exact Mass ]:
370.09000

[ PSA ]:
155.89000

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

Articles

New plate medium for screening and presumptive identification of gram-negative urinary tract pathogens.

J. Clin. Microbiol. 26 , 791-793, (1988)

A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. The medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucu...

Fluorogenic assay for rapid detection of Escherichia coli in food.

Appl. Environ. Microbiol. 50 , 1383-1387, (1985)

An assay procedure to screen for Escherichia coli in foods by using 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into lauryl tryptose (LST) broth was evaluated. The beta-glucuronidase pr...

R.B. Thompson, B. Richard

Anal. Lett. 18 , 1847, (1985)