PNGase F

Names

[ Name ]:
PNGase F

[Synonym ]:
glycopeptidase
glycopeptidase f
glycopeptidase a
n-glycanase(r)
n-glycosidase a
ec 3.5.1.52
n-glycanase
MFCD00131222
n-linked-glycopeptide-[n-acetyl-beta-d-glucosaminyl]-l-asparagine amidohydrolase

Biological Activity

[Description]:

PNGase F, a glycosidase, catalyzes the cleavage of an internal glycoside bond in an oligosaccharide. PNGase F removes nearly all N-linked oligosaccharides from glycoproteins[1][2].

[Related Catalog]:

Research Areas >> Cancer

[In Vitro]

PNGase F 可以在糖分析工作流程中从糖蛋白中释放 N-聚糖[1]。

[References]

[1]. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7.

[2]. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204.

Chemical & Physical Properties

[ Storage condition ]:
20°C

Safety Information

[ Symbol ]:

GHS05, GHS06, GHS09

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H302-H311-H317-H318-H410

[ Precautionary Statements ]:
P273-P280-P305 + P351 + P338-P312-P501

[ Personal Protective Equipment ]:
Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)

[ Hazard Codes ]:
Xi,N,Xn

[ Risk Phrases ]:
37/38-41-50/53-43-20/21/22

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
2

Articles

The characterization and quantitation of glycomic changes in CHO cells during a bioreactor campaign.

Biotechnol. Bioeng. 109(12) , 3007-17, (2012)

Within the biotechnology industry there is a continuous drive to better and more fully understand the biopharmaceutical process in order to achieve better process control. A method to monitor and quan...

N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.

FEBS Lett. 588(1) , 111-6, (2014)

Recently, we disclosed that KIAA1199-mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin-coated vesicles or early endosomes), but no information about...

Liver and muscle hemojuvelin are differently glycosylated.

BMC Biochem. 12 , 52, (2011)

Hemojuvelin (HJV) is one of essential components for expression of hepcidin, a hormone which regulates iron transport. HJV is mainly expressed in muscle and liver, and processing of HJV in both tissue...

Related Compounds

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