Phenosafranine

Names

[ CAS No. ]:
81-93-6

[ Name ]:
Phenosafranine

[Synonym ]:
Phenazinium, 3,7-diamino-5-phenyl-, chloride (1:1)
Phenosafranin
3,7-diamino-5-phenyl phenazinium chloride
MFCD00036335
Phenosafranine
3,7-Diamino-5-phenylphenazin-5-ium chloride
EINECS 201-387-0
Phenosofranine
Safrinin B Extra
Phenosafrinine
Phenosofrinine
Schultz No. 958
Safranin B Extra
10-phenylphenazin-10-ium-2,8-diamine,chloride
Phenosafranine,chloride

Biological Activity

[Description]:

Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Widholm JM. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94.

[2]. Liu, W., et al. Characterization of Phenosafranine–Hemoglobin Interactions in Aqueous Solution. J Solution Chem 40, 231–246 (2011).

[3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016 May;86:345-51.

Chemical & Physical Properties

[ Density]:
1.1738 (rough estimate)

[ Boiling Point ]:
478.49°C (rough estimate)

[ Melting Point ]:
300ºC

[ Molecular Formula ]:
C₁₈H₁₅ClN₄

[ Molecular Weight ]:
322.792

[ Exact Mass ]:
322.098511

[ PSA ]:
68.81000

[ LogP ]:
0.99550

[ Index of Refraction ]:
1.6110 (estimate)

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1630000

CHEMICAL NAME :: Phenazinium, 3,7-diamino-5-phenyl-, chloride

CAS REGISTRY NUMBER :: 81-93-6

LAST UPDATED :: 199612

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C18-H15-N4.Cl

MOLECULAR WEIGHT :: 322.82

WISWESSER LINE NOTATION :: T C666 BK INJ BR& EZ MZ &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 112 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 18 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TEST SYSTEM :: Insect - Drosophila melanogaster

DOSE/DURATION :: 1000 ppm

REFERENCE :: AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
26-36

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
SG1630000

Articles

Spectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide.

Indian J. Biochem. Biophys. 35(4) , 241-6, (1998)

Interaction of Klebsiella K14 capsular polysaccharide with cationic dyes pinacyanol chloride, acridine orange and phenosafranin has been studied by spectrophotometric and spectrofluorometric technique...

Sulfite Oxidase Catalyzes Single-Electron Transfer at Molybdenum Domain to Reduce Nitrite to Nitric Oxide.

Antioxid. Redox Signal. 23 , 283-94, (2015)

Recent studies suggest that the molybdenum enzymes xanthine oxidase, aldehyde oxidase, and mARC exhibit nitrite reductase activity at low oxygen pressures. However, inhibition studies of xanthine oxid...

A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell.

Analyst 137(9) , 2233-8, (2012)

A new type of dehydrogenase-based amperometric glucose biosensor was constructed using glucose dehydrogenase (GDH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified ...