PD 404182

PD 404182 is a potent and competitive inhibitor of human dimethylarginine dimethylaminohydrolase 1 (DDAH1), with an IC50 of 9 μM. PD 404182 exhibits antiangiogenic and antiviral activity in vitro[1][2].

Signaling Pathways >> Others >> Others

Research Areas >> Inflammation/Immunology

IC50: 9 μM (DDAH1)[1]

PD 404182 (20 μM) increases asymmetric dimethylarginine (ADMA) approximately 70% in ECs[1]. PD 404182 (50-100 μM; 18 hours) attenuates endothelial tube formation in virto and does not perturb cell membrane integrity or induces cytotoxicity[1]. PD 404182 inhibits HIV-1 in seminal plasma, with an IC50 of 1 μM[2]. PD 404182 (300 μM;30 minutes) exhibits low toxicity toward several human cell lines , freshly activated PBMCs, primary CD4+ T lymphocytes, macrophages, and dendritic cells, and lactobacilli found in the normal vaginal flora[2]. Cell Viability Assay[1] Cell Line: Human dermal microvascular endothelial cells (ECs) Concentration: 10-300 μM Incubation Time: 24 hours Result: Did not induce cytotoxicity.

[1]. Ghebremariam YT, et, al. A novel and potent inhibitor of dimethylarginine dimethylaminohydrolase: a modulator of cardiovascular nitric oxide. J Pharmacol Exp Ther. 2014 Jan; 348(1): 69-76.

[2]. Chamoun-Emanuelli AM, et, al. Evaluation of PD 404,182 as an anti-HIV and anti-herpes simplex virus microbicide. Antimicrob Agents Chemother. 2014; 58(2): 687-97.