5-Ethynyl uridine

Names

[ Name ]:
5-Ethynyl uridine

[Synonym ]:
1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
5-ETHYNYL URIDINE

Biological Activity

[Description]:

5-Ethynyluridine (5-EU) is a potent cell-permeable nucleoside can be used to label newly synthesized RNA. 5-Ethynyluridine can be used for isolation and sequencing of nascent RNA from neuronal populations in vivo. 5-Ethynyluridine can be used to identify changes in transcription in vivo in nervous system disease models[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vivo]

5-Ethynyluridine (75 mM in PBS for 1 µl; intracerebellar injection ) shows a labeling of central nervous system cells in mouse[1]. 5-Ethynyluridine (330-750 µMol/g; i.p.) results in in labelling of nascent RNA in cells of multiple organs and tissues, but not in the central nervous system in mouse[1].

[References]

[1]. Van't Sant LJ, et al. In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol Commun. 2021 May 21;9(1):94.

[2]. Jao CY, et al. Exploring RNA transcription and turnover in vivo by using click chemistry. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15779-84.

Chemical & Physical Properties

[ Molecular Formula ]:
C₁₁H₁₂N₂O₆

[ Molecular Weight ]:
268.22300

[ Exact Mass ]:
268.07000

[ PSA ]:
124.78000

Synthetic Route

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Precursor & DownStream

Precursor

DownStream

Related Compounds

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