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(S)-(-)-Blebbistatin

Names

[ CAS No. ]:
674289-55-5

[ Name ]:
(S)-(-)-Blebbistatin

[Synonym ]:
(−)-Blebbistatin
(+/-)-Blebbistatin
(3aS)-3a-Hydroxy-6-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one
Blebbistatin
4H-Pyrrolo[2,3-b]quinolin-4-one, 1,2,3,3a-tetrahydro-3a-hydroxy-6-methyl-1-phenyl-, (3aS)-
(S)-Blebbistatin
3a-hydroxy-6-methyl-1-phenyl-2,3-dihydropyrrolo[2,3-b]quinolin-4-one

Biological Activity

[Description]:

Blebbistatin is a selective non-muscle myosin II (NMII) inhibitor, promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function[1].

[Related Catalog]:

Signaling Pathways >> Cytoskeleton >> Myosin
Research Areas >> Others

[Target]

Non-muscle myosin II (NMII)[1]


[In Vitro]

The therapeutic potential of targeting NMII to enhance CEC migration is investigated using bovine corneal endothelial cells (BCECs). Blebbistatin, a direct myosin motor inhibitor, promotes migration and directional persistence in CECs through decreasing actin retrograde flow and increasing lamellipodial protrusion persistence to accelerate wound healing in vitro[1].

[In Vivo]

Blebbistatin (0.05 mL, 20 μM; intracameral injection; daily; for 6 days; New Zealand white rabbits) treatment promotes wound healing in rabbit corneal endothelial scraping model[1]. Animal Model: New Zealand white rabbits (16-20 weeks; 3-3.5 kg) with injected xylazine 5 mg/kg and ketamine 40 mg/kg[1] Dosage: 0.05 mL; 20 μM Administration: Intracameral injection; daily; for 6 days Result: Resulted in significant improvement of corneal clarity and corneal edema resolution, implying the restoration of an intact corneal endothelial monolayer.

[References]

[1]. Ho WT, et al. Targeting non-muscle myosin II promotes corneal endothelial migration through regulating lamellipodial dynamics. J Mol Med (Berl). 2019 Sep;97(9):1345-1357.

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Boiling Point ]:
507.3±60.0 °C at 760 mmHg

[ Melting Point ]:
210-212ºC

[ Molecular Formula ]:
C18H16N2O2

[ Molecular Weight ]:
292.332

[ Flash Point ]:
260.6±32.9 °C

[ Exact Mass ]:
292.121185

[ PSA ]:
52.90000

[ LogP ]:
1.62

[ Vapour Pressure ]:
0.0±1.4 mmHg at 25°C

[ Index of Refraction ]:
1.681

Safety Information

[ Hazard Codes ]:
Xn

[ Risk Phrases ]:
R20/21/22

[ Safety Phrases ]:
26-36/37

Related Compounds