Cearoin

Cearoin, isolated from Dalbergia odorifera, increases autophagy and apoptosis through the production of ROS and the activation of ERK[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Protein Tyrosine Kinase/RTK >> ROS

Signaling Pathways >> Autophagy >> Autophagy

Signaling Pathways >> MAPK/ERK Pathway >> ERK

Signaling Pathways >> Stem Cell/Wnt >> ERK

ERK

ROS

Autophagy

Apoptosis

Cearoin (10-80 μM) induces cell death in a dose-dependent manner[1]. Cearoin (10-80 μM) increases the phosporylation of ERK in SH-SY5Y cells[1]. Cearoin (5-80 μM) increases the conversion of LC3B-I to LC3B-II in SH-SY5Y cells. The expression of LC3B-II is a good marker for autophagosome formation in the autophagy process[1]. Cell Viability Assay[1] Cell Line: Human neuroblastoma SH-SY5Y cells Concentration: 0, 1, 5, 10, 20, 40, or 80 μM Incubation Time: 6 or 12 hours Result: Significantly decreased cell viability from 10 μM in a dose-dependent manner. Treatment with 40 μM for 12 h induced about 50% loss in cell viability in SH-SY5Y cells. Western Blot Analysis[1] Cell Line: Human neuroblastoma SH-SY5Y cells Concentration: 0, 5, 10, 20, 40, or 80 μM Incubation Time: 12 hours Result: Increased ERK phosphorylation in a dose-dependent manner, whereas it did not alter JNK phosphorylation. Induced the formation of LC3B-II in a dose dependent manner.

[1]. Tonking Bastola, et al. Cearoin Induces Autophagy, ERK Activation and Apoptosis via ROS Generation in SH-SY5Y Neuroblastoma Cells. Molecules. 2017 Feb 6;22(2):242.