5-Hydroxy-2'-deoxyuridine
Names
[ CAS No. ]:
5168-36-5
[ Name ]:
5-Hydroxy-2'-deoxyuridine
[Synonym ]:
Uridine,2'-deoxy-5-hydroxy
2'-Deoxy-5-hydroxyuridine
Oh-5-du
Biological Activity
[Description]:
5-Hydroxy-2'-deoxyuridine (5-OHdU) is a major stable oxidation product of 2'-Deoxycytidine. 5-Hydroxy-2'-deoxyuridine can be incorporated into DNA in vitro by DNA polymerase[1].
[Related Catalog]:
[Target]
Human Endogenous Metabolite
[In Vitro]
To study the specificity of nucleotide incorporation opposite 5-hydroxypyrimidines in template DNA, 18- and 45-member oligodeoxyribonucleotides, containing an internal 2'-deoxy-5-hydroxyuridine (5-OHdU) in two different sequence contexts, were used. Translesion synthesis past 2'-deoxy-5-hydroxyuridine (5-OHdU) in both oligonucleotides occurred, but pauses both opposite, and one nucleotide prior to, the modified base in the template is observed. The specificity of nucleotide incorporation opposite 2'-deoxy-5-hydroxyuridine (5-OHdU) in the template is sequence context dependent. In one sequence context, dA is the principal nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). However in a second sequence context, dC is the predominant base incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU). In that same sequence context, dC is also the predominant nucleotide incorporated opposite 2'-deoxy-5-hydroxyuridine (5-OHdU)[1].
[References]
Chemical & Physical Properties
[ Density]:
1.698g/cm3
[ Molecular Formula ]:
C9H12N2O6
[ Molecular Weight ]:
244.20100
[ Exact Mass ]:
244.07000
[ PSA ]:
124.78000
[ Index of Refraction ]:
1.654
[ Storage condition ]:
2-8°C