Phenol Red sodium salt

Names

[ Name ]:
Phenol Red sodium salt

[Synonym ]:
Phenolsulfonephthalein sodium salt
Phenol Red,sodium salt
PR sodium salt
phenolsulfonphthalein sodium salt
phenolsulphophthaleine sodium salt
UNII-50J9732WBG
MFCD00066901
Phenol, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, monosodium salt
EINECS 252-057-8
phenolsulfonephthalein sodium
Sodium 4-(3-(4-hydroxyphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)phenolate
Phenolate, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, sodium salt (1:1)
phenol red sodium salt
PHENOL RED,ACS
Sodium Phenol Red
sodium hydrogen 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bisphenolate S,S-dioxide
Phenol Red (sodium salt)

Biological Activity

[Description]:

Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Phenol Red sodium salt is commonly employed as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminants will cause a change in pH resulting in a change in indicator color. Phenol red bears a structural resemblance to some nonsteroidal estrogens and has significant estrogenic activity at the concentrations (15-45 μM) at which it is found in tissue culture media. Phenol red binds to the estrogen receptor of MCF-7 human breast cancer cells and stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells[1]. Phenol red is a simple, rapid and inexpensive method for the measurement of hydrogen peroxide produced by cells in culture. The assay is based on the horseradish peroxidase-mediated oxidation of phenol red by hydrogen peroxide which results in the formation of a compound demonstrating increased absorbance at 610 nm[2].

[Cell Assay]

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

[References]

[1]. Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500.

[2]. Pick E, et al. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161-70.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
0.972

[ Boiling Point ]:
562.8ºC at 760mmHg

[ Melting Point ]:
285 °C (dec.)(lit.)

[ Molecular Formula ]:
C₁₉H₁₃NaO₅S

[ Molecular Weight ]:
375.351

[ Flash Point ]:
294.2ºC

[ Exact Mass ]:
375.030853

[ PSA ]:
95.04000

[ LogP ]:
4.62760

[ Storage condition ]:
Store at RT.

[ Stability ]:
Stable. Incompatible with strong oxidizing agents.

[ Water Solubility ]:
soluble

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi:Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36-S37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
29349990

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 29349990

Related Compounds

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