M5N36

M5N36 is a potent and selective Cdc25C inhibitor with IC50 values of 0.15, 0.19, 0.06 µM for Cdc25A, Cdc25B, Cdc25C, respectively. M5N36 shows anti-proliferative activity and increases the expression of p-CDK1 and p-CDK2[1].

Research Areas >> Cancer

Signaling Pathways >> Metabolic Enzyme/Protease >> Phosphatase

IC50: 0.15 µM (Cdc25A); 0.19 µM (Cdc25B); 0.06 µM (Cdc25C)[1]

M5N36 (5, 10 µM; 24 h) 增加 MDA-MB-231 细胞中 p-CDK1 和 p-CDK2 的表达[1]。 Cell Proliferation Assay[1] Cell Line: Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells Concentration: Incubation Time: Result: Showed anti-proliferative activity with IC50s of 2.88, 3.57, 10.00, 2.00, 1.20, 6.03, 3.39, 2.40 µM for Eca-109, HepG2, MCF-TAM, MDA-MB-231, Raji, SGC-7901, SW-620, HUVEC cells, respectively. Western Blot Analysis[1] Cell Line: MDA-MB-231 cells Concentration: 5, 10 µM Incubation Time: 24 h Result: Increased the expression of Cdc25A, Cdc25B, increased the expression of p-CDK1 and p-CDK2.

[1]. Tao Y, et al. Discovery of potent and selective Cdc25 phosphatase inhibitors via rapid assembly and in situ screening of Quinonoid-focused libraries. Bioorg Chem. 2021 Oct;115:105254.