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Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

Names

[ CAS No. ]:
210344-99-3

[ Name ]:
Ac-Trp-Glu-His-Asp-AFC trifluoroacetate salt

[Synonym ]:
AFC156
Ac-WEHD-AFC
N-Acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine
Ac-Trp-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin
L-α-Asparagine, N-acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
Ac-Trp-Glu-His-Asp-AFC

Biological Activity

[Description]:

Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1].

[Related Catalog]:

Research Areas >> Cancer
Signaling Pathways >> Others >> Others
Research Areas >> Inflammation/Immunology

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase-1 Fluorogenic Activity Assay[1]: 1. Incubate the cells according to your normal protocol. 2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
1351.5±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C38H37F3N8O11

[ Molecular Weight ]:
838.743

[ Flash Point ]:
771.1±34.3 °C

[ Exact Mass ]:
838.253418

[ PSA ]:
312.23000

[ LogP ]:
1.81

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.648

Related Compounds