4,5-Diaminofluorescein diacetate

Names

[ CAS No. ]:
205391-02-2

[ Name ]:
4,5-Diaminofluorescein diacetate

[Synonym ]:
5,6-Diaminofluorescein diacetate
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-5,6-diamino-
(6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate
5,6-Diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
DAF 2DA

Biological Activity

[Description]:

DAF 2DA is most widely probe for NO measurement.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Others

Dye Reagents

[In Vitro]

Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).

[References]

[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
719.0±60.0 °C at 760 mmHg

[ Molecular Formula ]:
C₂₄H₁₈N₂O₇

[ Molecular Weight ]:
446.409

[ Flash Point ]:
320.0±29.2 °C

[ Exact Mass ]:
446.111389

[ PSA ]:
140.17000

[ LogP ]:
1.20

[ Vapour Pressure ]:
0.0±2.3 mmHg at 25°C

[ Index of Refraction ]:
1.721

[ Storage condition ]:
2-8℃

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Risk Phrases ]:
R36/38

[ Safety Phrases ]:
26

[ RIDADR ]:
NONH for all modes of transport

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