HKOCl-4

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid with excellent sensitivity and selectivity[1]. HKOCl-4 has longer absorption wavelength and better pH stability compared with fluorescein-based probes. Ex: 530 nm; Em 557 nm.

Research Areas >> Others

Signaling Pathways >> Others >> Others

Ex: 530 nm; Em 557 nm

HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1]. HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of •OH and ONOO− as well as 10 equiv of O2, NO, 1O2, ROO•, TBHP and H2O2)[2]. HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 µM in RAW264.7 macrophages[2].

[1]. Dan Yang, et al. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033.

[2]. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues.