Ac-Asp-Glu-Val-Asp-AFC

Names

[ CAS No. ]:
201608-14-2

[ Name ]:
Ac-Asp-Glu-Val-Asp-AFC

[Synonym ]:
N-Acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine
L-α-Asparagine, N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
Ac-DEVD-AFC
MFCD01310970

Biological Activity

[Description]:

Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).

[Related Catalog]:

Signaling Pathways >> Others >> Others

Research Areas >> Cancer

Dye Reagents

Peptides

[In Vitro]

After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1].

[Cell Assay]

For the detection of caspase-3 activity, PBS washes cell pellets (derive from either the medium or the adherent cells) which are suspended in extract buffer [25 mM HEPES (pH7.4), 0.1% TritonX-l00, 10% glycerol, 5 mM DTT, 1mM phenylmethylsulfonyl fluoride, 10 mg/mL pepstatin, and 10 mg/mL Leupeptin] and vortexed vigorously. 20μl of extract (corresponding to 10% of the sample) are incubated with the caspase-3 fluorogenic substrates Ac-DEVD-AFC at 100 μM final concentration at room temperature, and caspase-3 activity is measured continuously by monitoring the release of fluorigenic AFC at 37°C[1].

[References]

[1]. Wang X, et al. Involvement of Bim in Photofrin-mediated photodynamically induced apoptosis. Cell Physiol Biochem. 2015;35(4):1527-36.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
1152.7±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C₃₀H₃₄F₃N₅O₁₃

[ Molecular Weight ]:
729.612

[ Flash Point ]:
650.9±34.3 °C

[ Exact Mass ]:
729.210510

[ PSA ]:
287.61000

[ LogP ]:
1.58

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.575

[ Storage condition ]:
−20°C

[ Water Solubility ]:
H2O: 1 mg/mL

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

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Related Compounds

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