DPPH

Names

[ Name ]:
DPPH

[Synonym ]:
DPPH radical
diphenylpicrylhydrazylfreeradical
2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl
DPPH
di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium
1,1-Diphenyl-2-picrylhydrazyl Free Radical
2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazino
Hydrazinyl, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-
Diphenylpicrylhydrazyl.
1,1-Diphenyl-2-picrylhydrazyl radical
2,2-Diphenylpicrylhydrazyl
2,2-diphenyl-1-picrylhydrazyl
1-diphenyl-2-picrylhydrazyl
EINECS 217-591-8
Diphenypicrylhydrazyl
MFCD00007231

Biological Activity

[Description]:

DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the radical scavenging activity of antioxidants. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine. DPPH method may be utilized in aqueous and nonpolar organic solvents and can be used to examine both hydrophilic and lipophilic antioxidants[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption[1]. DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines[1].

[References]

[1]. Kedare SB, et al. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011 Aug;48(4):412-22.

Chemical & Physical Properties

[ Melting Point ]:
136-138ºC

[ Molecular Formula ]:
C₁₈H₁₂N₅O₆

[ Molecular Weight ]:
394.318

[ Exact Mass ]:
394.078766

[ PSA ]:
140.70000

[ LogP ]:
5.97000

[ Appearance of Characters ]:
green

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
Soluble in dimethyl formamide, ethanol, chloroform, ether, carbon disulfinde, acetone, oils, carbon tetrachloride, glacial acetic acid and toluene. Insoluble in water.

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: MW3250000

CHEMICAL NAME :: Hydrazyl, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-

CAS REGISTRY NUMBER :: 1898-66-4

BEILSTEIN REFERENCE NO. :: 1846081

LAST UPDATED :: 199701

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C18-H12-N5-O6

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 1800 ug/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: CSLNX* U.S. Army Armament Research & Development Command, Chemical Systems Laboratory, NIOSH Exchange Chemicals. (Aberdeen Proving Ground, MD 21010) Volume(issue)/page/year: NX#06207

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H317-H334

[ Precautionary Statements ]:
P261-P280-P342 + P311

[ Hazard Codes ]:
Xn:Harmful;

[ Risk Phrases ]:
R20/21/22

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
UN2811

[ WGK Germany ]:
1

[ RTECS ]:
MW3250000

[ Packaging Group ]:
II

[ Hazard Class ]:
6.1

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Related Compounds

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