Mito-TRFS

Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells[1].

Research Areas >> Cancer

Signaling Pathways >> Others >> Others

Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C[1]. Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm[1]. Immunofluorescence[1]. Cell Line: HeLa cells. Concentration: 1 μM. Incubation Time: 2 h. Result: Mito-TRFS is localized predominantly in the mitochondria of live cells.

[1]. Yaping Liu, et al. A small molecule probe reveals declined mitochondrial thioredoxin reductase activity in a Parkinson's disease model. Chem Commun (Camb). 2016 Feb 7;52(11):2296-9.