Axinelline A

Axinelline A is a potent COX inhibitor with IC50s of 2.22 μM and 8.89 μM against COX-2 and COX-1, respectively. Axinelline A shows anti-inflammatory activity[1].

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Immunology/Inflammation >> COX

COX-2:2.22 μM (IC50)

COX-1:8.89 μM (IC50)

Axinelline A (2-30 μM; 24 h) 抑制脂多糖 (LPS; HY-D1056) 诱导的 RAW264.7 细胞中促炎因子 (NO、TNF-α、IL-6、IL-1β 和 PGE2) 的表达[1]。 Axinelline A (2-30 μM; 24 h) 抑制 LPS 诱导的 RAW264.7 细胞 NF-κB 信号通路[1]。 Western Blot Analysis[1] Cell Line: RAW264.7 cells Concentration: 2, 10 and 30 μM Incubation Time: 24 h Result: Diminished LPS-induced expression of nitric oxide synthase (iNOS) and COX-2 protein levels. The phosphorylation level of NF-κB increased after 30 min of LPS treatment, but pretreatment with test compound reduced the level of phosphorylation in a dose-dependent manner. The phosphorylation of IKK and IκBα was inhibited in a dose-dependent manner.

[1]. Ju Z, et al. Synthesis and Anti-Inflammatory Activity of the Natural Cyclooxygenase-2 Inhibitor Axinelline A and Its Analogues. J Nat Prod. 2023 Apr 28;86(4):958-965.