Resorufin butyrate

Names

[ Name ]:
Resorufin butyrate

[Synonym ]:
(7-oxophenoxazin-3-yl) butanoate

Biological Activity

[Description]:

Resorufin butyrate is a fluorogenic substrate for triglyceride lipases and cholinesterase (Ex=570 nm, Em=580 nm)[1][2][3].

[Related Catalog]:

Research Areas >> Metabolic Disease

Signaling Pathways >> Neuronal Signaling >> AChE

[Target]

Triglyceride lipases, cholinesterase[1]

[In Vitro]

Resorufin butyrate (2.3 μM) displays a linear increase in fluorescence over 60 min (conducted in 384-well plates using 12 nM of bLPL enzyme and substrate in PBS, pH 7.5, 5% DMSO)[1].

[References]

[1]. J Biomol Screen, et al. Resorufin butyrate as a soluble and monomeric high-throughput substrate for a triglyceride lipase. J Biomol Screen. 2012 Feb;17(2):245-51.

[2]. Guilbault, G.G., et al. Resorufin butyrate and indoxyl acetate as fluorogenic substrates for chloinesterase. Anal Chem. 1965 Jan;37:120-3.

[3]. J.Hofmann, et al. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry : Resorufin-β-d-galactopyranoside as a New Fluorogenic Substrate for β-Galactosidase. Anal. Chim. Acta 163, 67-72 (1984).

Chemical & Physical Properties

[ Density]:
1.31g/cm3

[ Boiling Point ]:
428.7ºC at 760 mmHg

[ Melting Point ]:
136-138ºC(lit.)

[ Molecular Formula ]:
C₁₆H₁₃NO₄

[ Molecular Weight ]:
283.27900

[ Flash Point ]:
194.5ºC

[ Exact Mass ]:
283.08400

[ PSA ]:
69.40000

[ LogP ]:
2.99830

[ Vapour Pressure ]:
1.48E-07mmHg at 25°C

[ Index of Refraction ]:
1.62

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Mapping substrate selectivity of lipases from thermophilic fungi

J. Mol. Catal., B Enzym. 49 , 104-112, (2007)

Related Compounds

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