Okadaic acid ammonium salt

Names

[ Name ]:
Okadaic acid ammonium salt

[Synonym ]:
MFCD00153856
okadaic acid ammonium salt

Biological Activity

[Description]:

Okadaic acid, a marine toxin, is an inhibitor of protein phosphatases (PP), including PP1 (IC50=15-50 nM), PP2A (IC50=0.1-0.3 nM), PP3 (IC50=3.7-4 nM), PP4 (IC50=0.1 nM), PP5 (IC50=3.5 nM), with a significantly higher affinity for PP2A. Okadaic acid increase of phosphorylation of a number of proteins by inhibiting PP, and acts a tumor promoter[1] [2].

[Related Catalog]:

Research Areas >> Cancer

Signaling Pathways >> Metabolic Enzyme/Protease >> Phosphatase

Research Areas >> Neurological Disease

[In Vitro]

Okadaic acid inhibits the proliferation of AGS, MNK-45, Caco 2 cells [3].Okadaic acid (10 nM, 8 hours) increases Drp1 phosphorylation and mitochondrial fission in rat cortical neurons [4]. Cell Proliferation Assay[3] Cell Line: AGS, MNK-45 and Caco 2 cell lines Concentration: 0-100 nM Incubation Time: 24 h or 48 h Result: Inhibited the proliferation of AGS, MNK-45, Caco 2 cells.

[In Vivo]

Okadaic acid induces Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection [5]. Animal Model: Female wild-type C57BL/6 mice (6 to 8 months)[5] Dosage: 100 μM Administration: injected unilaterally to the lateral amygdala Result: Induced Tau phosphorylation and protein aggregation in anatomically distinct brain regions 24 h post-injection.

[References]

[1]. [1] Kleppe R, et al. Cell Death Inducing Microbial Protein Phosphatase Inhibitors--Mechanisms of Action. Mar Drugs. 2015 Oct 22;13(10):6505-20.

[2]. [2] Valdiglesias V, et al. Okadaic acid: more than a diarrheic toxin. Mar Drugs. 2013 Oct 31;11(11):4328-49.

[3]. [3] del Campo M, et al. Okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines. Mar Drugs. 2013;11(12):4751-4760.

[4]. [4] Cho MH, et al. Increased phosphorylation of dynamin-related protein 1 and mitochondrial fission in okadaic acid-treated neurons. Brain Res. 2012 May 15;1454:100-10.

Chemical & Physical Properties

[ Boiling Point ]:
947.3ºC at 760 mmHg

[ Molecular Formula ]:
C₄₄H₇₁NO₁₃

[ Molecular Weight ]:
822.03

[ Flash Point ]:
526.7ºC

[ Exact Mass ]:
821.49300

[ PSA ]:
185.66000

[ LogP ]:
4.25510

[ Vapour Pressure ]:
0mmHg at 25°C

[ Storage condition ]:
20°C

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS06

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H301 + H311 + H331-H315

[ Precautionary Statements ]:
Missing Phrase - N15.00950417-P261-P280-P302 + P352 + P312-P304 + P340 + P312-P403 + P233

[ Hazard Codes ]:
T: Toxic;

[ Risk Phrases ]:
R23/24/25

[ Safety Phrases ]:
22-26-36/37/39-45

[ RIDADR ]:
UN 2811 6.1 / PGIII

[ WGK Germany ]:
3