MAPTAM

MAPTAM is an intracellular Ca2+ chelator[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Incubate the cells according to your normal protocol. 2. Pre-incubate cells for 30 minutes with 20 μM MAPTAM. 3. Replace medium with HEPES buffer containing 2 μM fura 2 pentaacetoxymethyl ester and 1% BSA (wt:vol). 4. Perform measurements in HEPES buffer containing 1 mM CaCl2.

[1]. Seewald S, et al. Role of mitogen-activated protein kinase in the angiotensin II-induced DNA synthesis in vascular smooth muscle cells. Hypertension. 1998 May;31(5):1151-6.