L-Azidonorleucine hydrochloride

L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2].

Research Areas >> Others

Signaling Pathways >> Others >> Others

CHO cells transfected with pMaRSC and incubated with L-Azidonorleucine (Anl; 1.5 mM; 6 h) are fixed, permeabilized, and treated with alkyne-TAMRA. The TAMRA fluorescence exclusively in the mCherry-expressing cells is observed, consistent with cell-selective incorporation of Anl into cellular proteins[1].

When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired[2].

[1]. Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81.

[2]. Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575.