ML 334
Names
[ CAS No. ]:
1432500-66-7
[ Name ]:
ML 334
Biological Activity
[Description]:
ML334 is a potent, cell permeable activator of NRF2 by inhibition of Keap1-NRF2 protein-protein interaction. ML334 binds to Keap1 Kelch domain with a Kd of 1 μM. ML334 stimulates NRF2 expression and nuclear translocation and induces antioxidant response elements (ARE) activity[1][2].
[Related Catalog]:
[Target]
Ki: 1 μM (Keap1)[2]
[In Vitro]
ML334 (LH601A; 50-100 µM; 6-16 hours; HEK293 cells) treatment increases NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. And also enhances HO-1 mRNA expression between 4- and 7-fold at 6 h, with lesser fold-changes at 16 h[1]. ML334 (LH601A; 50-100 µM; 16 hours; HEK293 cells) treatment induces HO-1 and TRX1 proteins in HEK293 cells[1]. ML334 (LH601A) stimulates NRF2 expression and nuclear translocation in HEK293 cells[1]. RT-PCR[1] Cell Line: HEK293 cells Concentration: 50 μM or 100 µM Incubation Time: 6 hours, 16 hours Result: Increased NQO1 and TRX1 mRNAs between 2- and 3-fold at both 6 and 16 h. Enhanced HO-1 mRNA expression between 4- and 7-fold at 6 h. Western Blot Analysis[1] Cell Line: HEK293 cells Concentration: 50 μM or 100 µM Incubation Time: 16 hours Result: Enhanced HO-1 and TRX1 protein expression at 16 h.
[References]
Chemical & Physical Properties
[ Density]:
1.354±0.06 g/cm3(Predicted)
[ Boiling Point ]:
693.1±55.0 °C(Predicted)
[ Molecular Formula ]:
C26H26N2O5
[ Molecular Weight ]:
446.50