SS28

SS28, a SRT501 analog with oral bioavailability, inhibits tubulin polymerization to cause cell cycle arrest at G2/M phase. SS28 results in apoptosis rather than necrosis tubulin[1].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> Microtubule/Tubulin

Signaling Pathways >> Cytoskeleton >> Microtubule/Tubulin

Tubulin[1].

SS28 (0-20 μM) induces cytotoxicity in different cancer cell lines[1]. SS28 treatment (5 μM for A549 and 2 μM for CEM) results in cell cycle arrest at G2/M phase leading to apoptosis upon further incubation[1]. Cell Proliferation Assay[1] Cell Line: CEM, Reh, Nalm6, SUDHL8, Molt4, A549, HeLa 293T cells. Concentration: 1, 5, 10 and 20 μM. Incubation Time: 48 and 72 h. Result: CEM and A549 exhibited maximum sensitivity to SS28 followed by SUDHL8, Molt4 and Reh, whereas Nalm6 showed moderate sensitivity after 48 h of the treatment. IC50 values of SS28 in CEM and A549 cell line were 2.6 and 5.2 μM, respectively, whereas in SUDHL8, Molt4, Reh and Nalm6 were 2.7, 5.1, 7.9 and 21 μ M, respectively after 48 h of treatment. Cell Cycle Analysis[1] Cell Line: CEM and A549 cells. Concentration: 2 μM (CEM) and 5 μM (A549). Incubation Time: 6, 12, 18, 24 and 30 h. Result: Showed significant accumulation of cells at G2/M phase in a time-dependent manner (6 to 24 h) and a subsequent increase in the sub-G1 population, indicative of apoptosis at 30 h in case of A549. Similarly, studies in CEM also showed a distinct G2/M arrest after 12 h of treatment, as compared to vehicle control.

SS28 (15 mg/kg. b.wt.) treatment results in inhibition of tumor cell proliferation[1]. Animal Model: BALB/c mice using EAC cells[1]. Dosage: 15 mg/kg. Administration: Orally on every alternate day for 9 doses. Result: There was no further tumor progression in the mice when SS28 was administered, unlike the untreated tumor control mice.

[1]. Thomas E, et al. A Novel Resveratrol Based Tubulin Inhibitor Induces Mitotic Arrest and Activates Apoptosis in Cancer Cells. Sci Rep. 2016 Oct 17;6:34653.