5(6)-carboxynaphthofluorescein

Names

[ Name ]:
5(6)-carboxynaphthofluorescein

[Synonym ]:
5(6)-Carboxynaphthofluorescein

Biological Activity

[Description]:

5(6)-Carboxynaphthofluorescein is a pH-dependent fluorophore. 5(6)-Carboxynaphthofluorescein shows good sensitivity in an alkaline pH range and it can be exploited in the construction of fiber-optic pH sensors. 5(6)-Carboxynaphthofluorescein can be used as a fluorescent pH indicator (Ex/Em=593/668 nm)[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

5(6)-Carboxynaphthofluorescein can be used as a fluorescent pH indicator, it is sensitive in the pH range from 6.6 to 8.6 with a pKa value of 7.6[1]. 5(6)-Carboxynaphthofluorescein have fluorescence properties, with excitation wavelength=593 nm, emission spectrum=668 nm[1].

[References]

[1]. Nevolova S, et al. Development of Fluorescent Assay for Monitoring of Dehalogenase Activity. Biotechnol J. 2019 Mar;14(3):e1800144.

Chemical & Physical Properties

[ Molecular Formula ]:
C₂₉H₁₆O₇

[ Molecular Weight ]:
476.43300

[ Exact Mass ]:
476.09000

[ PSA ]:
125.04000

[ LogP ]:
6.02160

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Colorful methods to detect ion channels and pores: intravesicular chromogenic probes that respond to pH, pM and covalent capture.

Org. Biomol. Chem. 7 , 1784-1792, (2009)

The activity of synthetic pores, ion channels, transporters and carriers is usually determined with fluorescent probes in vesicles or by conductance measurements in planar lipid bilayers. Elaborating ...

High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye.

Anal. Chem. 69 , 863-867, (1997)

A fast and durable ratiometric pH microoptode that is highly accurate, precise, sensitive, reversible, and reproducible over the physiological ranges of pH, ionic strength, and temperature has been de...

Array biosensor based on enzyme kinetics monitoring by fluorescence spectroscopy: application for neurotoxins detection.

Biosens. Bioelectron. 22 , 3001-3007, (2007)

The aim of the present work is to develop an evanescence wave array biosensor exploiting the "kinetic" approach of enzymatic reaction and further detection of the reaction products via pH sensitive fl...