BAPTA tetrasodium

Names

[ Name ]:
BAPTA tetrasodium

[Synonym ]:
1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrasodium salts (BAPTA Na)
BAPTA tetrasodium salt hydrate
BAPTA,Tetrasodium salt
BAPTA tetrasodium
Tetrasodium 2,2',2'',2'''-[1,2-ethanediylbis(oxy-2,1-phenylenenitrilo)]tetraacetate
Tetrasodium 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate
MFCD02683087
1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrasodium salt
1,2-Bis(2-Aminophenoxy)-Ethane-N,N,N,N-Tetraacetic Acid Tetrasodium Salt
BAPTA, TETRASODIUM SALT
tetrasodium,2-[2-[2-[2-[bis(carboxylatomethyl)amino]phenoxy]ethoxy]-N-(carboxylatomethyl)anilino]acetate

Biological Activity

[Description]:

BAPTA tetrasodium is a selective chelator for calcium. BAPTA tetrasodium, as calcium indicator, has high selectivity against magnesium and calcium. BAPTA tetrasodium is widely used as an intracellular buffer for investigating the effects of Ca2+ release from intracellular stores or influx via Ca2+-permeable channels in the plasma membrane. BAPTA tetrasodium can also inhibit phospholipase C activity independently of their role as Ca2+ chelators[1][2][3].

[Related Catalog]:

Signaling Pathways >> Metabolic Enzyme/Protease >> Phospholipase

Research Areas >> Others

[In Vitro]

BAPTA (0.3-30 μM; 1 h) can be used for the prevention of [Ca2+]-induced cell damage, but disturbe calcium signalling in isingle differentiated NH15-CA2 neuroblastoma and glioma hybrid cells[3]. BAPTA (0-10 mM) inhibits phospholipase C (PLC) activity in a dose-dependent manner, and is unrelated to Ca2+[2].

[References]

[1]. R Y Tsien, et al. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 1980 May 27;19(11):2396-404.

[2]. Roger C Hardie, et al. Inhibition of phospholipase C activity in Drosophila photoreceptors by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA) and di-bromo BAPTA. Cell Calcium. 2005 Dec;38(6):547-56.

[3]. M B Collatz, et al. Intracellular calcium chelator BAPTA protects cells against toxic calcium overload but also alters physiological calcium responses. Cell Calcium

Chemical & Physical Properties

[ Boiling Point ]:
766.6ºC at 760 mmHg

[ Molecular Formula ]:
C₂₂H₂₀N₂Na₄O₁₀

[ Molecular Weight ]:
564.361

[ Flash Point ]:
417.4ºC

[ Exact Mass ]:
564.070862

[ PSA ]:
185.46000

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xn

[ Risk Phrases ]:
R20/21/22

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

Articles

New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures.

Biochemistry 19 , 2396, (1980)

A new family of high-affinity buffers and optical indicators for Ca2+ is rationally designed and synthesized. The parent compound is 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), a...