LEISHMAN'S STAIN

Names

[ Name ]:
LEISHMAN'S STAIN

[Synonym ]:
EINECS 235-732-1
MFCD00131498

Biological Activity

[Description]:

Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Prepare stock solution of Sorensen’s buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4 (9.1 g/L). 2. Cover the smear with Leishman stain for 2 min. 3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min. 4. Wash off with running water and dry the smear (5 min).

[References]

[1]. S Gajendra, et al. Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int J Lab Hematol. 2015 Dec;37(6):774-82.

Chemical & Physical Properties

[ Molecular Formula ]:
n_.a_.

[ Appearance of Characters ]:
Powder

[ Storage condition ]:
Store at +15°C to +30°C.

[ Water Solubility ]:
methanol: soluble1mg/mL | Soluble in water (4 mg/ml), methanol (1 mg/ml), ethanol (4 mg/ml), and methyl cellosolve (20 mg/ml).

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
36/38-36/37/38

[ Safety Phrases ]:
S22;S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Note on a Simple and Rapid Method of Producing Romanowsky Staining in Malarial and other Blood Films.

Br. Med. J. 2 , 757-8, (1901)

Evaluation of different methods for diagnosis of P. falciparum malaria.

Indian J. Med. Microbiol. 24 , 49-51, (2006)

Rapid diagnosis is a prerequisite for institution of effective treatment and reducing the mortality and morbidity of falciparum malaria. This study was taken up to compare the efficacy of various rapi...

Related Compounds

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