StemRegenin 1 (SR1)
Names
[ CAS No. ]:
1227633-49-9
[ Name ]:
StemRegenin 1 (SR1)
[Synonym ]:
4-(2-{[2-(1-Benzothiophen-3-yl)-9-isopropyl-9H-purin-6-yl]amino}ethyl)phenol
Phenol, 4-[2-[[2-benzo[b]thien-3-yl-9-(1-methylethyl)-9H-purin-6-yl]amino]ethyl]-
StemRegenin1
StemRegenin 1
SR1
Biological Activity
[Description]:
StemRegenin 1 is a potent aryl hydrocarbon receptor (AhR) antagonist with IC50 of 127 nM.
[Related Catalog]:
[Target]
[In Vitro]
StemRegenin 1 (SR1) acts by antagonizing the aryl hydrocarbon receptor (AhR). StemRegenin 1 increases the number of CD34+ cells after 5 to 7 days with an EC50 of ~120 nM. StemRegenin 1 inhibits photoaffinity ligand (PAL) binding (IC50=40 nM) These results support the conclusion that StemRegenin 1 -induced CD34+ cell expansion is mediated through direct binding and inhibition of the AhR[1]. An aryl hydrocarbon receptor antagonist, StemRegenin 1 (SR1), robustly promotes ex vivo expansion of human CD34+ cells. StemRegenin 1 treatment accelerates the proliferation of CD34+ cells and decreases the expression levels of VentX[2].
[Cell Assay]
A quantity of 250,000 CB-derived CD34+ cells are cultured with control conditions (DMSO, 0.01%) or StemRegenin 1 (0.75 μM) for 3 weeks. At this point control cultures had expanded 1080-fold and StemRegenin 1 treated cells expanded 2024-fold relative to starting cell numbers. A quantity of 30 to 30,000 uncultured CD34+ CB-derived cells or a fraction of the final culture equivalent to 30 to 10,000 starting cells are transplanted. The cells are injected intravenously via the retro-orbital route into sub-lethally irradiated (300 rads, 200 rads) 6- to 10-week-old NSG mice. Engraftment is performed within 24 h after irradiation. Engraftment is monitored by flow cytometric analysis of blood obtained via retro-orbital sinus or bone marrow using anti-human CD45 and anti-mouse CD45 antibodies. The mice are sacrificed between 13-16 weeks posttransplantation; bone marrow (from both femurs and tibiae), spleen and thymus are collected for analysis. For secondary engraftment, 50% of the bone marrow from each recipient mouse is transplanted into one secondary sub-lethally irradiated NSG mouse. Fifteen weeks after transplantation, bone marrow is harvested from the secondary mice and analyzed by flow cytometry[1].
[References]
[Related Small Molecules]
Chemical & Physical Properties
[ Density]:
1.4±0.1 g/cm3
[ Boiling Point ]:
622.9±65.0 °C at 760 mmHg
[ Molecular Formula ]:
C24H23N5OS
[ Molecular Weight ]:
429.537
[ Flash Point ]:
330.5±34.3 °C
[ Exact Mass ]:
429.162323
[ PSA ]:
104.10000
[ LogP ]:
5.10
[ Appearance of Characters ]:
white solid
[ Vapour Pressure ]:
0.0±1.9 mmHg at 25°C
[ Index of Refraction ]:
1.721
[ Storage condition ]:
+2C to +8C
Safety Information
[ Hazard Codes ]:
Xn
[ Risk Phrases ]:
22