Ganoderic acid Mk

Ganoderic acid Mk (GA-Mk) is a triterpenoid acid, that can be isolated from the mycelia of Ganoderma lucidum. Ganoderic acid Mk is efficiently anti-proliferative and can induce apoptosis of HeLa cells by mitochondria-mediated pathway. Ganoderic acid Mk can be used for cervical cancer research[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Apoptosis >> Caspase

Signaling Pathways >> Metabolic Enzyme/Protease >> MMP

Caspase-3

Caspase-9

Ganoderic acid Mk (0-100 μM, 24 h) 对人类癌细胞表现出比人类正常细胞系更强的细胞毒性[1]。 Ganoderic acid Mk (0-40 μM, 12-48 h) 对HeLa细胞增殖具有剂量依赖性抑制作用[1]。 Ganoderic acid Mk (0-40 μM, 24 h) 诱导HeLa细胞凋亡[1]。 Ganoderic acid Mk (0-40 μM, 24 h) 诱导 ROS 爆发，MMP 减少和 caspase-3, -9 活性增加[1]。 Cell Cytotoxicity Assay[1] Cell Line: Human normal cell lines (MCF-10A and HF) and cancer cell lines (HO-8910PM, SW1990, 95-D and HeLa) Concentration: 0-100 μM Incubation Time: 24 h Result: Exhibited stronger cytotoxicity to human cancer cells (IC50 values were ranged within 29.8-44.2 µM) than human normal cell lines (IC50 values to MCF-10A and HF were 84.5 µM and 78.4 µM, respectively). Cell Viability Assay[1] Cell Line: HeLa cells Concentration: 0, 5, 10, 20, 40 μM Incubation Time: 12, 24, 48 h Result: Showed cytotoxicity on HeLa cells in a dose- and time-dependent manner. Apoptosis Analysis[1] Cell Line: HeLa cells Concentration: 0, 10, 20, 40 μM Incubation Time: 24 h Result: Increased the rate of early and late apoptotic cells in a dose-dependent manner in HeLa cells. GA-Mk could induce HeLa cells apoptosis in parallel with the accumulation of ROS, the loss of MMP and the activation of activities of caspases.

[1]. Liu R M, et al. Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells[J]. Latin American Journal of Pharmacy, 2012, 31(1):43-50.

[2]. Ding N, et al. Separation and determination of four ganoderic acids from dried fermentation mycelia powder of Ganoderma lucidum by capillary zone electrophoresis. J Pharm Biomed Anal. 2010 Dec 15;53(5):1224-30.