LysoTracker Yellow HCK 123

LysoTracker Yellow HCK 123 is a potent yellow membrane-permeable fluorescent probe. LysoTracker Yellow HCK 123 is a weakly basic amine that selectively accumulates in cellular compartments with low luminal pH. (λex=465 nm, λem=535 nm)[1].

Research Areas >> Others

Signaling Pathways >> Others >> Others

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). LysoTracker Yellow HCK 123 assay[1] (to quantify GAPDH): 1. Culture EMT6 cells in 8-well cell culture slides. Incubate the cells according to your normal protocol. 2. Add TONS504 per well to make the final concentration at 30 µg/mL.Incubate cells at 37 °C for 4 hours. 3. Add LysoTracker Yellow HCK 123 per well to make the final concentration at 50 μM. Incubate cells at 37 °C, 5% CO2, for 30 minutes. 4. Analyze samples on a confocal laser scanning microscope.

[1]. Desclés J, et, al. New tools for labeling silica in living diatoms. New Phytol. 2008;177(3):822-829.

[2]. Desclés J, et, al. New tools for labeling silica in living diatoms. New Phytol. 2008;177(3):822-829.