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Kinetensin

Names

[ CAS No. ]:
103131-69-7

[ Name ]:
Kinetensin

[Synonym ]:
nrp
h-ile-ala-arg-arg-his-pro-tyr-phe-leu-oh
ile-ala-arg-arg-his-pro-tyr-phe-leu
iarrhpyfl
kinetensin
neurotensin-related peptide

Biological Activity

[Description]:

Kinetensin is a neurotensin-like peptide isolated from pepsin-treated human plasma.

[Related Catalog]:

Signaling Pathways >> GPCR/G Protein >> Neurotensin Receptor
Peptides
Research Areas >> Neurological Disease

[Target]

Neurotensin Receptor[1]


[In Vitro]

The peptide kinetensin isolated from pepsin-treated human plasma induces a dose-dependent release of histamine when exposed to rat peritoneal mast cells. The threshold concentration is around 1 μM, the ED50 is 10 μM, and the optimal concentration of between 100 to 1000 μM released 80% of the total histamine. Kinetensin is 10 to 100 times less potent than neurotensin and equipotent with the opioid peptide dynorphin. The histamine release is clearly temperature-dependent, with no release occurring at 0 or 45 °C and with an optimum around 37 °C. The histamine release is significantly reduced in the absence of extracellular calcium[2].

[In Vivo]

Kinetensin also induces a dose-dependent increase in vascular permeability when injected intradermally into rats[2].

[Cell Assay]

Rat peritoneal mast cells are incubated with kinetensin at 37°C for 10 min. The incubation is stopped by the addition of 1.8 mL of ice-cold buffered saline and cells are separated from supernatant by centrifugation. Histamine release is expressed as per cent of total mast cell histamine[2].

[Animal admin]

Rats[2] Anesthetized Sprague-Dawley rats are given 25I-albumin i.v. Samples are then injected intradermally in 5x2 spots on the back and comprised saline as a control or kinetensin in different doses in 100 μL saline. After 20 rain, skin biopsies of 7 mm diameter are cut out, weighed and transferred to a gamma-counter. Results are expressed as: (counts per min (cpm) in tissue per gram wet weight/cpm in plasma per mL plasma)[2].

[References]

[1]. Mogard MH, et al. The amino acid sequence of kinetensin, a novel peptide isolated from pepsin-treated human plasma: homology with human serum albumin, neurotensin and angiotensin. Biochem Biophys Res Commun. 1986 May 14;136(3):983-8.

[2]. Sydbom A, et al. Stimulation of histamine release by the peptide kinetensin. Agents Actions. 1989 Apr;27(1-2):68-71.


[Related Small Molecules]

ML314 | Neurotensin

Chemical & Physical Properties

[ Density]:
1.41 g/cm3

[ Molecular Formula ]:
C56H85N17O11

[ Molecular Weight ]:
1172.38000

[ Exact Mass ]:
1171.66000

[ PSA ]:
460.04000

[ LogP ]:
4.33470

[ Index of Refraction ]:
1.661

[ Storage condition ]:
2~8°C

Safety Information

[ Hazard Codes ]:
F+

[ Safety Phrases ]:
22-24/25

[ WGK Germany ]:
3.0

Related Compounds